NMR structural studies on membrane proteins

Application of Solution NMR to Structural Studies on α-Helical Integral Membrane Proteins.

A large portion of proteins in living organisms are membrane proteins which play critical roles in the biology of the cell, from maintenance of the biological membrane integrity to communication of cells with their surroundings. To understand their mechanism of action, structural information is essential. Nevertheless, structure determination of transmembrane proteins is still a challenging are...

Functional and Structural Studies on Heptahelical Membrane Proteins

12 Lyhennelmä (Synopsis in Finnish) 13

Structural studies of proteins by paramagnetic solid-state NMR spectroscopy.

Paramagnetism-based nuclear pseudocontact shifts and spin relaxation enhancements contain a wealth of information in solid-state NMR spectra about electron-nucleus distances on the ∼20 Å length scale, far beyond that normally probed through measurements of nuclear dipolar couplings. Such data are especially vital in the context of structural studies of proteins and other biological molecules th...

Structural Studies on Cytoskeletal Proteins.

A number of componenets of cytoskeleton are very large proteins which are in their complete native form non amenable to the structure determination via X-ray diffraction techniques due to their non-globular shape and intrinsic flexibility which hinder the formation of suitable crystals. Dissecting such proteins into their representative building blocks-domains and subsequent structural studies ...

Structural studies on lysosomal proteins